Home > Sandwich ELISA Kits > Human Vascular Endothelial Growth Factor Receptor 3 (VEGFR-3;FLT4) ELISA Kit

Product Name:

Human Vascular Endothelial Growth Factor Receptor 3 (VEGFR-3;FLT4) ELISA Kit

Cat.#:

JL15315

Regulatory Status:

RUO

Alternative:

FLT41; FLT4; PCL; Fms-Related Tyrosine Kinase 4; Tyrosine-protein kinase receptor FLT4

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.31-20ng/mL

Sensitivity:

0.12ng/mL

Specificity:

This kit recognizes Human VEGFR3；FLT4 in samples. No significant cross-reactivity or interference between Human VEGFR3；FLT4 and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| VEGFR3；FLT4

Vascular endothelial growth factor C (VEGF-C) is a protein that is a member of the platelet-derived growth factor / vascular endothelial growth factor (PDGF/VEGF) family. It is encoded in humans by the VEGFC gene, which is located on chromosome 4q34.The main function of VEGF-C is to promote the growth of lymphatic vessels (lymphangiogenesis). It acts on lymphatic endothelial cells (LECs) primarily via its receptor VEGFR-3 promoting survival, growth and migration.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.2	1.65	1.16	0.8	0.54	0.37	0.28	0.09
Corrected OD	2.11	1.56	1.07	0.71	0.45	0.28	0.19	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human VEGFR3；FLT4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	83-101	95
Plasma(n=8)	92-105	102
Tissue Homogenates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human VEGFR3；FLT4 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	84-96	88-97	90-110
1 : 2	Average (%)	92	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108